Circulating DNA kit

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Format:

BOX

Circulating DNA kit contiene reagenti per effettuare 24 test:

 

Nome Prodotto N° Test Conservazione Cat. No.
Circulating DNA kit 24 15~25℃ LCMBL03

Overview:

The Circulating DNA Kit provides silica-based membrane and special lysis buffer system for circulating DNA extraction effectively. This kit is specially designed for isolation and purification of DNA from peripheral blood (serum or plasma) and pleural effusion. The purified DNA is suitable for downstream applications such as real-time quantitative PCR (qPCR) and NGS.

The kit  incorporates absorbent spin columns which completely avoid the use of toxic and hazardous organic reagents and the overnight digestion with Proteinase K. DNA isolated with this kit is of high-quality and serves as an excellent template for gene mutation detection and gene expression analysis.

 

Storage:

The shelf life of the kit is 12 months, and should be stored dry at room temperature (15~25℃).

 

Assay Highlights:

Simple and quick (90 min)

4ml of plasma or serum sample

High purity and high yield

Good nucleic acid repair capacity

Good and stable PCR result

GMP-compliant manufacturing

ISO13485-certificated laboratory

CE-IVD

 

Principle and Procedure:

Peripheral blood and pleural effusion samples are lysed with specific buffer  and Proteinase K solution to release DNA. The lysate is mixed with isopropanol and DNA Tracer to collect DNA by centrifuging. Then the precipitates are dissolved in a specific buffer and ethanol to provide appropriate binding conditions for DNA, then the mixture is applied to a DNA spin column, where the DNA binds to the membrane and impurities are removed with wash buffer. The DNA is finally eluted in its buffer.

 

Cell-free DNA Protection Vacuum Tub

 

Intended useprovetta
10 ml EDTA-anticoagulant blood collection tube, which contains cell free DNA protection reagent for stabilization of cell free plasma DNA. The collected blood is stable for 7 days at 4~25°C.

 

Clinical significance
Blood collection tubes stabilize nucleated blood cells, preventing the release of cellular genomic DNA and cell free DNA, that maintain the tumor DNA content in plasma DNA, improve the gene mutation detection sensitivity and accuracy.

 

Plasma DNA amount validation

 

Same collected whole blood:

 

  • Plasma isolation and DNA extraction on 0, 1st, 3rd, 5th, 7th day.
  • Housekeeping gene of genomic DNA PCR testing with the purified DNA, that indicate the plasma DNA amount.
  • In the blood collection tubes, the Ct values of housekeeping gene are stable for 7 days, that show the cell-free DNA in that is stable.

 

 

grafico 1

 

 

EGFR mutant DNA amount validation

 

Same collected whole bloods, mixed with same EGFR mutant DNA:

 

  • Plasma isolation and DNA extraction on 0, 1st, 3rd, 5th, 7th day.
  • EGFR mutation PCR testing with the purified DNA, that indicate the EGFR mutant DNA amount in plasma.
  • In the blood collection tubes, the Ct values of housekeeping gene are stable for 7 days, that show the cell-free DNA in that is stable.

 

 

grafico 2

Contatto: info@lcm-genect.it

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